2QBV
Crystal Structure of Intracellular Chorismate Mutase from Mycobacterium Tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.860, 59.860, 47.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.330 - 2.000 |
| R-factor | 0.222 |
| Rwork | 0.219 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ybz |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.985 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.7 W8RSSI) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.330 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | 0.323 |
| Total number of observations | 10903 | |
| Number of reflections | 6192 | |
| <I/σ(I)> | 25.3 | 7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.7 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 295 | 20% polyethylene glycol 400, 0.1M tris pH 8.6, 0.2M magnesium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
