2QAZ
Structure of C. crescentus SspB ortholog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-08-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.07179 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 131.213, 131.213, 131.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.490 - 2.700 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.091 | 0.351 |
| Number of reflections | 20900 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.2 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | 1.3 M (NH4)2SO4, 250 mM NaCl, 100mM Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
