2QAS
Crystal structure of Caulobacter crescentus SspB ortholog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-11-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97908 |
| Spacegroup name | P 64 |
| Unit cell lengths | 121.760, 121.760, 27.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.760 - 2.550 |
| R-factor | 0.218 |
| Rwork | 0.213 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.074 | 0.377 |
| Number of reflections | 7817 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 98.0 | 85.2 |
| Redundancy | 10.3 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | 1.4 M (NH4)2SO4, 0.1 M Tris, pH 8.0, hanging drop, temperature 300K, VAPOR DIFFUSION, HANGING DROP |
