2QAR

Structure of the 2TEL crystallization module fused to T4 lysozyme with a helical linker.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 32
Unit cell lengths	119.512, 119.512, 58.133
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	103.700 - 2.400
R-factor	0.222
Rwork	0.220
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.121
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	150.000	150.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.070	0.046	0.397
Number of reflections	35939
<I/σ(I)>	17.3
Completeness [%]	99.2	99.5	98
Redundancy	6.3	8.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1