2QA5
Crystal structure of Sept2 G-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.960, 53.920, 110.270 |
| Unit cell angles | 90.00, 128.37, 90.00 |
Refinement procedure
| Resolution | 19.790 - 3.400 |
| R-factor | 0.28144 |
| Rwork | 0.280 |
| R-free | 0.31391 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.589 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.340 | |
| Number of reflections | 10431 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 98.2 | |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 5% PEG 6000, 0.1M BICINE, pH 9.0, 0.1M Glycine, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
