2Q9X

Crystal structure of highly stable mutant Q40P/S47I/H93G of human fibroblast growth factor-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-20
Detector	MAR CCD 165 mm
Wavelength(s)	0.86980
Spacegroup name	P 21 21 21
Unit cell lengths	34.610, 57.599, 57.716
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.600 - 1.700
R-factor	0.209
Rwork	0.207
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rg8
RMSD bond length	0.015
RMSD bond angle	1.533
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.610	1.790
High resolution limit [Å]	1.600	1.700
Rmerge	0.097	0.356
Total number of observations		7487
Number of reflections	13265
<I/σ(I)>	5.2	1.9
Completeness [%]	100.0	100
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	15% PEG 8000, 100mM TrisHCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K