2Q9T
High-resolution structure of the DING protein from Pseudomonas fluorescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9535 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.728, 123.679, 40.833 |
| Unit cell angles | 90.00, 116.71, 90.00 |
Refinement procedure
| Resolution | 61.900 - 1.430 |
| R-factor | 0.133 |
| Rwork | 0.131 |
| R-free | 0.16400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Human Phosphate-binding protein (HPBP) PDB code 2cap |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.119 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.900 | 1.500 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Number of reflections | 59063 | |
| <I/σ(I)> | 38.9 | 10.52 |
| Completeness [%] | 98.1 | 89.2 |
| Redundancy | 4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 24% PEG 8000, 100 mM acetate buffer pH 4.5, 200 mM Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
