2Q9O
Near-atomic resolution structure of a Melanocarpus albomyces laccase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.740, 61.900, 124.210 |
| Unit cell angles | 90.00, 96.36, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.300 |
| R-factor | 0.1674 |
| Rwork | 0.167 |
| R-free | 0.21240 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.028 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 310642 | |
| <I/σ(I)> | 11.4 | 3 |
| Completeness [%] | 96.7 | 91.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | 15% PMME 2000, 0.2 M ammonium sulphate, 0.1 M sodium acetate , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
