2Q9L
Crystal structure of iMazG from Vibrio DAT 722: Ctag-iMazG (P43212)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-25 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 88.246, 88.246, 159.107 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.270 - 2.200 |
R-factor | 0.219 |
Rwork | 0.218 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2q73 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.210 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.171 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.117 | 0.578 |
Number of reflections | 30948 | |
<I/σ(I)> | 4 | 1.2 |
Completeness [%] | 94.8 | 71.6 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.05 | 293 | 0.1 M sodium citrate, 1.9 M ammonium sulfate, 500 mM NaCl, 10% 2-methyl-2,4-pentanediol, 10 mM MgCl2, pH 5.05, VAPOR DIFFUSION, SITTING DROP, temperature 293K |