2Q9L

Crystal structure of iMazG from Vibrio DAT 722: Ctag-iMazG (P43212)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-25
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 43 21 2
Unit cell lengths	88.246, 88.246, 159.107
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.270 - 2.200
R-factor	0.219
Rwork	0.218
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2q73
RMSD bond length	0.011
RMSD bond angle	1.210
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.171	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.117	0.578
Number of reflections	30948
<I/σ(I)>	4	1.2
Completeness [%]	94.8	71.6
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.05	293	0.1 M sodium citrate, 1.9 M ammonium sulfate, 500 mM NaCl, 10% 2-methyl-2,4-pentanediol, 10 mM MgCl2, pH 5.05, VAPOR DIFFUSION, SITTING DROP, temperature 293K