2Q97
Complex of mammalian actin with toxofilin from toxoplasma gondii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 54.523, 54.523, 363.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.23139 |
| Rwork | 0.229 |
| R-free | 0.28429 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a3z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.305 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.091 | 0.268 |
| Number of reflections | 19198 | |
| <I/σ(I)> | 38.6 | 14 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 34.3 | 27.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 277 | 8% PEG 4000, 10% GLYCEROL, 0.1M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, temperature 277K |
