2Q8X
The high-resolution crystal structure of ixt6, a thermophilic, intracellular xylanase from G. stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2001-11-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.481, 80.579, 79.054 |
| Unit cell angles | 90.00, 91.89, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.450 |
| R-factor | 0.1499 |
| R-free | 0.19010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1R85 Initial model of Xylanase T-6 (family10) of G.bacillus stearothermophilus |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.444 | |
| Number of reflections | 171850 | |
| <I/σ(I)> | 18.1 | 1.5 |
| Completeness [%] | 91.6 | 52.3 |
| Redundancy | 7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.9M sodium acetate, 0.1M cacodylate pH=6.5, 293.0 K, VAPOR DIFFUSION, HANGING DROP |
