2Q8V

NblA protein from T. vulcanus crystallized with urea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97913
Spacegroup name	P 32 2 1
Unit cell lengths	43.265, 43.265, 149.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.500
Rwork	0.245
R-free	0.26400
Structure solution method	SAD/Molecular Replacement
Starting model (for MR)	1ojh
RMSD bond length	0.010
RMSD bond angle	1.090
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CCP4
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.073	0.326
Number of reflections	6108
<I/σ(I)>	19.1	4.4
Completeness [%]	99.8	100
Redundancy	6.2	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	25% elthelene glycol, 2M Urea, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8	293	25% elthelene glycol, 2M Urea, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K