2Q8O
crystal structure of mouse GITR ligand dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-08 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.556, 70.402, 71.647 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.190 - 1.750 |
| R-factor | 0.18036 |
| Rwork | 0.180 |
| R-free | 0.22085 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.405 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.190 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 27551 | |
| <I/σ(I)> | 48.56 | 8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.9 | 21.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 100 mM HEPES pH7.5 and 20% PEG10000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
