2Q8D

Crystal structure of JMJ2D2A in ternary complex with histone H3-K36me2 and succinate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	200
Detector technology	CCD
Collection date	2007-02-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 2
Unit cell lengths	100.857, 148.642, 57.346
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.800 - 2.290
R-factor	0.20602
Rwork	0.204
R-free	0.25057
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gp5
RMSD bond length	0.011
RMSD bond angle	1.294
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.800	2.380
High resolution limit [Å]	2.290	2.290
Rmerge	0.090	0.471
Number of reflections	38447
<I/σ(I)>	36.1	2.7
Redundancy	4.7	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1