2Q8B

Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-09-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	184.905, 51.487, 95.257
Unit cell angles	90.00, 108.49, 90.00

Refinement procedure

Resolution	27.950 - 2.300
R-factor	0.21152
Rwork	0.209
R-free	0.25565
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z40 2cgr
RMSD bond length	0.008
RMSD bond angle	1.070
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	36116
<I/σ(I)>	14	4
Completeness [%]	99.5	98.4
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	294	20 mM MES, 10 mM MnCl2, 6 % PEG 3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K