2Q87

The Crystal Structure of the Human IRp60 Ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	200
Detector technology	CCD
Collection date	2006-12-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	50.490, 61.240, 106.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.800 - 1.700
R-factor	0.218
Rwork	0.215
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nms
RMSD bond length	0.018
RMSD bond angle	1.862
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.800	22.800
High resolution limit [Å]	1.700	1.700
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	VAPOR DIFFUSION, HANGING DROP, temperature 293.15K