2Q83
Crystal structure of ytaA (2635576) from Bacillus subtilis at 2.50 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97904, 0.97932 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 172.999, 172.999, 192.574 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.961 - 2.500 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.21000 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.655 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.961 | 29.960 | 2.560 |
| High resolution limit [Å] | 2.500 | 11.180 | 2.500 |
| Rmerge | 0.126 | 0.051 | 0.864 |
| Total number of observations | 6665 | 47319 | |
| Number of reflections | 59155 | ||
| <I/σ(I)> | 4.6 | 10.5 | 0.9 |
| Completeness [%] | 99.9 | 94.8 | 100 |
| Redundancy | 10.8 | 8.9 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.57 | 277 | NANODROP, 2.23M Ammonium sulfate, 0.1M Citric acid pH 5.57, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
