2Q7S

Crystal structure of N-formylglutamate amidohydrolase (YP_297560.1) from Ralstonia eutropha JMP134 at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97944, 0.97917
Spacegroup name	P 21 21 21
Unit cell lengths	58.610, 74.835, 145.163
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.604 - 2.000
R-factor	0.202
Rwork	0.199
R-free	0.24600
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.604
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.604	29.600	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.078	0.043	0.382
Total number of observations		1610	11021
Number of reflections	43945
<I/σ(I)>	6.9	13.5	2
Completeness [%]	99.9	93.5	99.9
Redundancy	3.5	3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	NANODROP, 30.0% PEG 6000, 0.1M MES pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K