2Q74
Mycobacterium tuberculosis SuhB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.400, 185.600, 107.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 2.600 |
| R-factor | 0.22372 |
| Rwork | 0.223 |
| R-free | 0.24471 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ima |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.259 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.068 | 0.258 |
| Number of reflections | 30931 | |
| <I/σ(I)> | 24.5 | 2.5 |
| Completeness [%] | 98.7 | 97.4 |
| Redundancy | 4.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
