2Q6Q
Crystal structure of Spc42p, a critical component of spindle pole body in budding yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.860, 57.790, 57.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.360 - 1.970 |
| R-factor | 0.21565 |
| Rwork | 0.212 |
| R-free | 0.29655 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | GCN4 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.908 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.020 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.075 | 0.370 |
| Number of reflections | 9799 | |
| <I/σ(I)> | 8.2 | 3.1 |
| Completeness [%] | 93.9 | 47 |
| Redundancy | 4.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.25 | 294 | 100 mM Tris, 200 mM cobalt chloride, 25% PEG 3350, pH 8.25, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
