2Q66
Structure of Yeast Poly(A) Polymerase with ATP and oligo(A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.633, 85.907, 107.469 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.710 - 1.800 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 2HHP |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.244 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.072 | 0.049 | 0.275 |
| Number of reflections | 56633 | ||
| <I/σ(I)> | 12.8 | ||
| Completeness [%] | 95.7 | 99.9 | 76 |
| Redundancy | 12 | 12 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 273 | .1M bis tris propane pH 6.4, .2M Li acetate, 16% Peg 3350, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | bis tris propane | ||
| 2 | 1 | 1 | Li acetate | ||
| 3 | 1 | 1 | Peg | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | bis tris propane | ||
| 6 | 1 | 2 | Li acetate | ||
| 7 | 1 | 2 | Peg |
