2Q60
Crystal structure of the ligand binding domain of polyandrocarpa misakiensis rxr in tetramer in absence of ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.260, 96.120, 151.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.220 - 2.900 |
| R-factor | 0.272 |
| Rwork | 0.269 |
| R-free | 0.32500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RXR part of PDB ENTRY 1XDK |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.003 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.224 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.071 | 0.409 |
| Number of reflections | 27210 | |
| <I/σ(I)> | 18.51 | 4.4 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 5.26 | 5.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M Pipes. 17% Peg 4K. 15mM Sodium chloride. 1mM n-dodecyl B-D-maltoside, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
