2Q5Z

Crystal structure of iMazG from Vibrio DAT 722: Ntag-iMazG (P43212)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-08-28
Detector	MAR scanner 345 mm plate
Spacegroup name	P 43 21 2
Unit cell lengths	63.333, 63.333, 163.227
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.170 - 2.300
R-factor	0.18
Rwork	0.179
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vmg
RMSD bond length	0.016
RMSD bond angle	1.453
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.173	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.099	0.496
Number of reflections	15523
<I/σ(I)>	39.9	9
Completeness [%]	99.8	100
Redundancy	32.1	32.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5	293	0.1 M sodium citrate, 8% PEG 8000, 0.1M NaCl, pH 5.50, VAPOR DIFFUSION, SITTING DROP, temperature 293K