2Q5X
Crystal Structure of the C-terminal domain of hNup98
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-19 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.10005 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 69.674, 69.674, 90.964 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.900 |
Rwork | 0.206 |
R-free | 0.22900 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.208 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | DM (4.2) |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.053 | 0.028 | 0.285 |
Number of reflections | 18307 | ||
<I/σ(I)> | 11.1 | ||
Completeness [%] | 99.1 | 94.6 | 99.9 |
Redundancy | 3 | 2.8 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 296 | 4.5M sodium formate, pH 4.6, vapor diffusion, hanging drop, temperature 296K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 296 | 4.5M sodium formate, pH 4.6, vapor diffusion, hanging drop, temperature 296K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 296 | 4.5M sodium formate, pH 4.6, vapor diffusion, hanging drop, temperature 296K |