2Q5H
Crystal structure of apo-wildtype Glycyl-tRNA synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93300 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 92.530, 92.530, 246.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.270 - 3.000 |
R-factor | 0.20749 |
Rwork | 0.205 |
R-free | 0.25013 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ati |
RMSD bond length | 0.007 |
RMSD bond angle | 1.004 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.186 | 0.986 |
Number of reflections | 21172 | |
<I/σ(I)> | 10.3 | 1.8 |
Completeness [%] | 95.6 | 90 |
Redundancy | 9.2 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% PEG 3350, 0.2M Sodium bromide, 0.1M Bis-Tris propane, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |