2Q5B
High resolution structure of Plastocyanin from Phormidium laminosum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 84.329, 84.329, 90.536 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.990 - 1.450 |
| R-factor | 0.19075 |
| Rwork | 0.190 |
| R-free | 0.21017 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1baw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.285 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (1.3.1) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.110 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.071 | 0.626 |
| Number of reflections | 57906 | |
| <I/σ(I)> | 25.4 | 2 |
| Completeness [%] | 99.6 | 96 |
| Redundancy | 9.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1 M NA CACODYLATE BUFFER (PH 6), 0.2 M ZN ACETATE, 15% PEG 8000 , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
