Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2005-02-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 141.122, 141.122, 105.253 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.310 - 3.500 |
| R-factor | 0.20079 |
| Rwork | 0.195 |
| R-free | 0.25547 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Abrin-a (PDB code: 1ABR) |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.725 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.310 | 3.700 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.095 | 0.539 |
| Number of reflections | 11360 | |
| <I/σ(I)> | 13.1 | 2.3 |
| Completeness [%] | 95.7 | 96.8 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 8000, TRIS-HCL BUFFER, PH 8.5, , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
