2Q3G
Structure of the PDZ domain of human PDLIM7 bound to a C-terminal extension from human beta-tropomyosin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9182 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.052, 55.404, 57.316 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.840 - 1.110 |
| R-factor | 0.13272 |
| Rwork | 0.131 |
| R-free | 0.15679 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homoly based model |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.690 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.840 | 1.150 |
| High resolution limit [Å] | 1.110 | 1.110 |
| Rmerge | 0.042 | 0.144 |
| Number of reflections | 58329 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 5.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M HEPES; 10% isopropanol; 20% PEG 4K, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
