2Q2L
Crystal Structure of Superoxide Dismutase from P. atrosanguina
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-19 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.140, 64.120, 63.620 |
| Unit cell angles | 90.00, 124.33, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.367 |
| Rwork | 0.167 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.427 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.428 |
| High resolution limit [Å] | 2.367 | 2.367 |
| Rmerge | 0.054 | 0.149 |
| Number of reflections | 10953 | |
| <I/σ(I)> | 32.7 | 6 |
| Completeness [%] | 94.4 | 63.6 |
| Redundancy | 6.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG 3350, 0.2 M Ammonium Iodide, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
