2Q2H
Crystal structure of the protein secretion chaperone CsaA from Agrobacterium tumefaciens with a genetically fused phage-display derived peptide substrate at the N-terminus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-10-27 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 61 |
Unit cell lengths | 60.528, 60.528, 115.247 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.860 - 1.650 |
R-factor | 0.161 |
Rwork | 0.160 |
R-free | 0.17300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gd7 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.183 |
Data reduction software | CrystalClear |
Data scaling software | d*TREK (8.0SSI) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.860 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.039 | 0.162 |
Total number of observations | 6400 | |
Number of reflections | 25681 | |
<I/σ(I)> | 30 | 8.6 |
Completeness [%] | 89.4 | 49.7 |
Redundancy | 5.71 | 4.47 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | 30% PEG 4000, 0.4 M ammonium acetate, 0.1 M sodium citrate pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |