2Q2G

Crystal structure of dimerization domain of HSP40 from Cryptosporidium parvum, cgd2_1800

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97926
Spacegroup name	C 1 2 1
Unit cell lengths	162.480, 60.590, 48.830
Unit cell angles	90.00, 106.19, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.2488
Rwork	0.249
R-free	0.29090
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.064	0.331
Number of reflections	34983
<I/σ(I)>	12.61	4.13
Completeness [%]	99.3	96.7
Redundancy	8	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	298	19% PEG 3350, 0.2 M LiSO4, 0.1 M Bis-Tris pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5.8	298	19% PEG 3350, 0.2 M LiSO4, 0.1 M Bis-Tris pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K