2Q24
Crystal structure of TetR transcriptional regulator SCO0520 from Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-12 |
| Detector | APS-1 |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 52.295, 146.152, 47.305 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22500 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.559 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.076 | 0.416 |
| Number of reflections | 34267 | |
| <I/σ(I)> | 33.658 | 3.2 |
| Completeness [%] | 99.6 | 95.4 |
| Redundancy | 5.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 273 | 0.1M Na(OAc) pH 4.0, 0.2M Ammonium acetate, 26% PEG 3350, 4% Sucrose, 2.5mM Tylosin tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
