2Q1Z
Crystal Structure of Rhodobacter sphaeroides SigE in complex with the anti-sigma ChrR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2004-08-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.28060 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 43.601, 119.640, 280.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.400 |
| R-factor | 0.28646 |
| Rwork | 0.284 |
| R-free | 0.32930 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.401 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.066 | 0.490 |
| Number of reflections | 29316 | |
| <I/σ(I)> | 32.64 | 5.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 11.75 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295.15 | sodium acetate, 1.6-1.9M sodium formate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
