2Q1H

Ancestral Corticoid Receptor in Complex with Aldosterone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.99
Spacegroup name	P 43 21 2
Unit cell lengths	79.658, 79.658, 113.871
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.270 - 1.900
R-factor	0.207
Rwork	0.207
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2q1v
RMSD bond length	0.009
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.270	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.037	0.370
Number of reflections	29610
<I/σ(I)>	63.8	7.5
Completeness [%]	99.9	100
Redundancy	10.1	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	295.15	9.5-15% PEG 3350, 5% glycerol, 50 mM Bis-Tris, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K