2Q19
2-keto-3-deoxy-D-arabinonate dehydratase apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 128.930, 128.930, 225.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.960 - 3.000 |
| R-factor | 0.21591 |
| Rwork | 0.214 |
| R-free | 0.24319 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2q18 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.064 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.116 | 0.381 |
| Number of reflections | 18610 | |
| <I/σ(I)> | 9.5 | 3.8 |
| Completeness [%] | 95.9 | 99.5 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | Na/K phosphate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
