2Q18
2-keto-3-deoxy-D-arabinonate dehydratase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97950 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 127.815, 127.815, 222.366 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 111.110 - 2.100 |
| R-factor | 0.20216 |
| Rwork | 0.201 |
| R-free | 0.21882 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.067 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.110 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.094 | 0.582 |
| Number of reflections | 53699 | |
| <I/σ(I)> | 23.1 | 3.4 |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | Na/K phosphate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
