2Q0Y

Crystal structure of GCN5-related N-acetyltransferase (YP_295895.1) from Ralstonia eutropha JMP134 at 1.80 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-30
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97891
Spacegroup name	P 32 2 1
Unit cell lengths	102.520, 102.520, 39.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.591 - 1.800
R-factor	0.144
Rwork	0.143
R-free	0.16500
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.344
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.591	29.591	1.860
High resolution limit [Å]	1.800	3.870	1.800
Rmerge	0.050	0.025	0.298
Number of reflections	21939	4093	3214
<I/σ(I)>	11.78	29.3	2.1
Completeness [%]	96.1	93.6	81.3
Redundancy	3.61

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	277	NANODROP, 0.2M Mg Formate, 20.0% PEG 3350, No Buffer pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 277K