2Q0V
Crystal structure of ubiquitin conjugating enzyme E2, putative, from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 101.474, 101.474, 83.871 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.400 |
| R-factor | 0.227 |
| Rwork | 0.224 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j74 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.806 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.099 | |
| Number of reflections | 11808 | |
| <I/σ(I)> | 7.8 | 2.9 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 20.8 | 20.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.4 M Na/KPi pH 7.0, 1 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
