2Q0O
Crystal structure of an anti-activation complex in bacterial quorum sensing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2005-06-09 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.127 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.900, 62.480, 65.500 |
| Unit cell angles | 94.87, 110.45, 99.17 |
Refinement procedure
| Resolution | 39.850 - 2.000 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l3l |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.088 | 0.464 |
| Number of reflections | 53940 | |
| <I/σ(I)> | 5.4 | 1.3 |
| Completeness [%] | 96.7 | 96.7 |
| Redundancy | 1.96 | 1.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1 mM DTT, 60 mM HEPES, 120 mM CaCl2, 16.8% PEG400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
