2Q0J
Structure of Pseudomonas Quinolone Signal Response Protein PqsE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.760, 66.090, 110.260 |
| Unit cell angles | 90.00, 97.19, 90.00 |
Refinement procedure
| Resolution | 19.820 - 2.100 |
| R-factor | 0.154 |
| Rwork | 0.151 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.731 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.820 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.071 | 0.357 |
| Number of reflections | 36642 | |
| <I/σ(I)> | 13.73 | 4.6 |
| Completeness [%] | 97.8 | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 100 mM imidazole, 0.6 M NaAc, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
