2Q0H

ABC Protein ArtP in complex with ADP/Mg2+, ATP-gamma-S hydrolyzed

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-07
Detector	MAR CCD 165 mm
Wavelength(s)	1.05000
Spacegroup name	P 1 21 1
Unit cell lengths	48.428, 84.007, 69.535
Unit cell angles	90.00, 105.60, 90.00

Refinement procedure

Resolution	50.000 - 2.200
Rwork	0.233
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ouk
RMSD bond length	0.007
RMSD bond angle	1.455
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	24166
<I/σ(I)>	27.28	3.97
Completeness [%]	88.5
Redundancy	3.6	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	20% PEG 3350, 0,2 M lithium cloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K