2Q0G
Terminal uridylyl transferase 4 from Trypanosoma brucei with bound UPU
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Collection date | 2006-11-25 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.544, 41.953, 106.593 |
Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
Resolution | 46.520 - 2.300 |
R-factor | 0.231 |
Rwork | 0.228 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ikf |
RMSD bond length | 0.013 |
RMSD bond angle | 1.608 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.2L) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.530 | 46.530 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.141 | 0.052 | 0.520 |
Total number of observations | 12172 | 11771 | |
Number of reflections | 32493 | ||
<I/σ(I)> | 5.6 | 16.2 | 2.1 |
Completeness [%] | 99.9 | 99.7 | 100 |
Redundancy | 3.65 | 3.53 | 3.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 100 mM sodium cacodylate, 200 mM calcium acetate, 18% PEG-8000, 4 mM MgCl2, 50 uM UTP, crystals soaked in 10 mM UPU for 30 minutes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |