2Q07
Crystal structure of AF0587, a protein of unknown function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.791, 58.745, 51.258 |
| Unit cell angles | 90.00, 96.91, 90.00 |
Refinement procedure
| Resolution | 32.040 - 2.040 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.28400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.040 | 0.430 |
| Number of reflections | 16046 | |
| <I/σ(I)> | 15 | 1 |
| Completeness [%] | 96.0 | 52 |
| Redundancy | 3 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2M Magnesium formate, 20% PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
