2Q06
Crystal structure of Influenza A Virus H5N1 Nucleoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97845 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 153.578, 153.578, 153.578 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.320 - 3.300 |
| R-factor | 0.206 |
| Rwork | 0.202 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iqh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.236 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.564 | 3.380 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.141 | 0.660 |
| Number of reflections | 18405 | |
| <I/σ(I)> | 11.16 | 2.1 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 0.15 M KCl, 0.01 M MgCl2, 0.1 M cacodylate pH 6.2, 7% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
