2Q01
Crystal structure of glucuronate isomerase from Caulobacter crescentus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97960 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 177.332, 190.176, 319.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.340 |
| R-factor | 0.19972 |
| Rwork | 0.198 |
| R-free | 0.25125 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1js5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.177 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.188 | 0.600 |
| Number of reflections | 112315 | |
| <I/σ(I)> | 2.7 | 0.4 |
| Completeness [%] | 92.7 | 61.9 |
| Redundancy | 2.5 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 294 | 1.0 M Sodium-potassium phosphate, pH 8.2, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
