2Q00
Crystal structure of the P95883_SULSO protein from Sulfolobus solfataricus. NESG target SsR10.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97909 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 87.927, 87.927, 176.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.030 - 2.400 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.26500 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.053 | 0.545 |
| Number of reflections | 25384 | |
| <I/σ(I)> | 26.8 | 2.6 |
| Completeness [%] | 97.4 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 6 | 277 | 20 % PEG 4000, 0.1 M Ammonium thiocyanate, 0.1 M MES, pH 6.0, MICROBATCH UNDER OIL, temperature 277K |
