2PZ9
Crystal structure of putative transcriptional regulator SCO4942 from Streptomyces coelicolor
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792, 0.9794 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 66.960, 66.960, 104.345 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.780 - 2.800 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.486 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.780 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.084 | 0.299 |
| Number of reflections | 7016 | |
| <I/σ(I)> | 46.985 | 5.3 |
| Completeness [%] | 99.6 | 96.4 |
| Redundancy | 10.7 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M Tris-HCl pH 8.5, 2.5M Ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
