2PZ0

Crystal structure of Glycerophosphodiester Phosphodiesterase (GDPD) from T. tengcongensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-05-27
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	49.401, 53.608, 110.885
Unit cell angles	90.00, 97.91, 90.00

Refinement procedure

Resolution	10.000 - 1.910
Rwork	0.229
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o1z
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.910	1.910
Rmerge	0.052	0.322
Number of reflections	43433
Completeness [%]	99.6	98.8
Redundancy	4.88

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M HEPES, 18% PEG4000, 10% iso-propanal, 0.05M CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K