2PYT
Crystal structure of a putative ethanolamine utilization protein q (eutq, stm2468) from salmonella typhimurium lt2 at 1.90 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-03 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97901, 0.97929 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.151, 39.043, 44.923 |
| Unit cell angles | 109.99, 104.79, 95.38 |
Refinement procedure
| Resolution | 28.490 - 1.900 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.22400 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.677 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.490 | 28.490 | 1.950 |
| High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
| Rmerge | 0.096 | 0.092 | 0.335 |
| Total number of observations | 363 | 2575 | |
| Number of reflections | 17651 | ||
| <I/σ(I)> | 3.4 | 5.1 | 0.8 |
| Completeness [%] | 97.0 | 92.8 | 96.3 |
| Redundancy | 2 | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | NANODROP, 2.4M (NH4)2SO4, 0.1M Bicine pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
