2PYK

Crystal structure of Staphylococcal nuclease variant V66Q/P117G/H124L/S128A at room temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OTHER
Temperature [K]	298
Detector technology	CCD
Collection date	2006-03-15
Detector	APEX II CCD
Spacegroup name	P 41
Unit cell lengths	48.967, 48.967, 63.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.203
Rwork	0.186
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Staphyloccocal nuclease V66E/P117G/H124L/S128A variant (at 100K) with b-factors set to 20.0 A^2 and residue 66 truncated to Ala waters excluded
Data reduction software	APEX
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.023	0.134
Number of reflections	8861
<I/σ(I)>	32.85	7.09
Completeness [%]	99.4	100
Redundancy	8.2	4.89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	277	37% MPD, 0.025 M potassium phosphate, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K